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Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification.
Li, Guotian; Jones, Kyle C; Eudes, Aymerick; Pidatala, Venkataramana R; Sun, Jian; Xu, Feng; Zhang, Chengcheng; Wei, Tong; Jain, Rashmi; Birdseye, Devon; Canlas, Patrick E; Baidoo, Edward E K; Duong, Phat Q; Sharma, Manoj K; Singh, Seema; Ruan, Deling; Keasling, Jay D; Mortimer, Jenny C; Loqué, Dominique; Bartley, Laura E; Scheller, Henrik V; Ronald, Pamela C.
Afiliação
  • Li G; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Jones KC; Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
  • Eudes A; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Pidatala VR; Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
  • Sun J; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Xu F; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Zhang C; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Wei T; Biomass Science and Conversion Technology Department, Sandia National Laboratories, CA94551, Livermore, USA.
  • Jain R; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Birdseye D; Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, 73019, USA.
  • Canlas PE; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Baidoo EEK; Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
  • Duong PQ; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Sharma MK; Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
  • Singh S; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Ruan D; Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
  • Keasling JD; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Mortimer JC; Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
  • Loqué D; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
  • Bartley LE; Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
  • Scheller HV; School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
  • Ronald PC; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
BMC Biotechnol ; 18(1): 54, 2018 09 04.
Article em En | MEDLINE | ID: mdl-30180895
ABSTRACT

BACKGROUND:

Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice.

RESULTS:

Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0 plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues.

CONCLUSION:

Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Aciltransferases / Plantas Geneticamente Modificadas / Lignina / Panicum Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Oryza / Aciltransferases / Plantas Geneticamente Modificadas / Lignina / Panicum Idioma: En Ano de publicação: 2018 Tipo de documento: Article