Continuous infusion of PTH1-34 delayed fracture healing in mice.
Sci Rep
; 8(1): 13175, 2018 09 04.
Article
em En
| MEDLINE
| ID: mdl-30181648
ABSTRACT
Hyperparathyroidism, which is increased parathyroid hormone (PTH) levels in the blood, could cause delayed or non-union of bone fractures. But, no study has yet demonstrated the effects of excess continuous PTH exposure, such as that seen in hyperparathyroidism, for fracture healing. Continuous human PTH1-34 (teriparatide) infusion using an osmotic pump was performed for stabilized tibial fractures in eight-week-old male mice to determine the relative bone healing process compared with saline treatment. Radiographs and micro-computed tomography showed delayed but increased calcified callus formation in the continuous PTH1-34 infusion group compared with the controls. Histology and quantitative histomorphometry confirmed that continuous PTH1-34 treatment significantly increased the bone callus area at a later time point after fracture, since delayed endochondral ossification occurred. Gene expression analyses showed that PTH1-34 resulted in sustained Col2a1 and reduced Col10a1 expression, consistent with delayed maturation of the cartilage tissue during fracture healing. In contrast, continuous PTH1-34 infusion stimulated the expression of both Bglap and Acp5 through the healing process, in accordance with bone callus formation and remodeling. Mechanical testing showed that continuously administered PTH1-34 increased the maximum load on Day 21 compared with control mice. We concluded that continuous PTH1-34 infusion resulted in a delayed fracture healing process due to delayed callus cell maturation but ultimately increased biomechanical properties.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fraturas da Tíbia
/
Calo Ósseo
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Consolidação da Fratura
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Teriparatida
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Conservadores da Densidade Óssea
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article