Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.
Sci Rep
; 8(1): 13343, 2018 09 06.
Article
em En
| MEDLINE
| ID: mdl-30190491
ABSTRACT
We have built a setup for 3D single molecule localisation microscopy (SMLM) where a very high resolution is achieved by, firstly, the use of two objectives instead of one and, secondly, minimizing optical aberrations by refractive index matching with a glycerol-water mixture as immersion medium in conjunction with glycerol-immersion objectives. Multiple optical paths of the microscope allow to switch between astigmatic and interferometric localisation along the optical axis, thus enabling a direct comparison of the performance of these localisation methods.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Água
/
Imagem Individual de Molécula
/
Glicerol
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article