Differential effects of ß-glucan on oxidative stress, inflammation and copper transport in two intestinal regions of large yellow croaker Larimichthys crocea under acute copper stress.
Ecotoxicol Environ Saf
; 165: 78-87, 2018 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-30193167
ABSTRACT
The aim of the present study was to evaluate investigate the effects of ß-glucan on oxidative stress, inflammation and copper transport in two intestinal regions of large yellow croaker under acute copper stress. Fish were injected with ß-glucan at a dose of 0 or 5â¯mgâ¯kg-1 body weight on 6, 4 and 2 days before exposed to 0 and 368⯵g Cu L-1 for 48â¯h. Biochemical indicators (MDA, Cu content, MTs protein levels, Cu/Zn-SOD, CAT and iNOS activities), gene expressions of oxidative stresses (Cu/Zn-SOD, CAT, Nrf2, MTs and MTF-1), inflammatory responses (NF-κB, iNOS, IL-1ß, IL-6 and TNF-α) and Cu transporters (ATP7A, ATP7B and CTR1) were determined. In the anterior intestine, ß-glucan increased MTs levels, activities of Cu/Zn-SOD, CAT and iNOS, mRNA levels of MTs, CAT, iNOS, ATP7A and ATP7B, and reduced Cu content and CTR1 gene expression to inhibite Cu-induced MDA. But ß-glucan had no effect on inflammatory gene expressions. In the mid intestine, ß-glucan increased activities of Cu/Zn-SOD and iNOS, mRNA levels of Cu/Zn-SOD, CAT and iNOS to maintain MDA content. However, unlike the anterior intestine, ß-glucan had no effect on Cu transporter gene expressions. Furthermore, transcription factors (Nrf2, NF-κB and MTF-1) paralleled with their target genes in the mid intestine, but no correlation was observed between NF-κB and IL-1ß and TNF-α gene expressions in the anterior intestine. In conclusion, our results unambiguously showed that ß-glucan induced oxidative stress, inflammation and copper transport were varied between the anterior and mid intestines of fish under Cu stress.
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Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
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Perciformes
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Estresse Oxidativo
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Cobre
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Beta-Glucanas
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Intestinos
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article