Overexpression of the Drosophila ATR homologous checkpoint kinase Mei-41 induces a G2/M checkpoint in Drosophila imaginal tissue.
Hereditas
; 155: 27, 2018.
Article
em En
| MEDLINE
| ID: mdl-30202398
ABSTRACT
BACKGROUND:
DNA damage generally results in the activation of ATM/ATR kinases and the downstream checkpoint kinases Chk1/Chk2. In Drosophila melanogaster, the ATR homologue meiotic 41 (mei-41) is pivotal to DNA damage repair and cell cycle checkpoint signalling. Although various mei-41 mutant alleles have been analyzed in the past, no gain-of-function allele is yet available. To fill this gap, we have generated transgenic flies allowing temporal and tissue-specific induction of mei-41.RESULTS:
Overexpression of mei-41 in wing and eye anlagen affects proliferation and a G2/M checkpoint even in the absence of genomic stress. Similar consequences were observed following the overexpression of the downstream kinase Grapes (Grp) but not of Loki (Lok), encoding the respective Drosophila Chk1 and Chk2 homologues, in agreement with their previously reported activities. Moreover, we show that irradiation induced cell cycle arrest was prolonged in the presence of ectopic mei-41 expression. Similar to irradiation stress, mei-41 triggered the occurrence of a slower migrating form of Grp, implying specific phosphorylation of Grp in response to either signal. Using a p53R-GFP biosensor, we further show that overexpression of mei-41 was sufficient to elicit a robust p53 activation in vivo.CONCLUSION:
We conclude that overexpression of the Drosophila ATR homologue mei-41 elicits an effectual DNA damage response irrespective of irradiation.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Proteínas Serina-Treonina Quinases
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Proteínas de Ciclo Celular
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Proteínas de Drosophila
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Drosophila melanogaster
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Pontos de Checagem do Ciclo Celular
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article