A rapid cell-permeating turn-on probe for sensitive and selective detection of sulfite in living cells.
Org Biomol Chem
; 16(37): 8318-8324, 2018 09 26.
Article
em En
| MEDLINE
| ID: mdl-30206621
ABSTRACT
A rapid cell-permeating probe NJUXJ-1 was introduced for sensitive and selective detection of sulfite in living cells. It generated a turn-on response to sulfite with high sensitivity (detection limit 13.0 nM) and selectivity (at a physiological level) and low toxicity. The fluorescence of the detecting system was steady for a wide pH range (5-8) and a long period of time (over 12 h). The most attractive point, its rapid cell-permeating ability, made it suitable for bioimaging with a 2 min incubation time and shortened the whole detecting period (cell-permeation and reaction), and thus could decrease background interference. It offered a convenient approach for determining exogenous or endogenous sulfite levels in living cells and further applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sulfitos
/
Limite de Detecção
/
Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article