Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase <sup>th</sup> dG-tC FRET Pair.

Han, Ji Hoon; Park, Soyoung; Hashiya, Fumitaka; Sugiyama, Hiroshi

Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase ^th dG-tC FRET Pair.

Han, Ji Hoon; Park, Soyoung; Hashiya, Fumitaka; Sugiyama, Hiroshi.

Afiliação

Han JH; Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Park S; Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Hashiya F; Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Sugiyama H; Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.

Chemistry ; 24(64): 17091-17095, 2018 Nov 16.

Article em En | MEDLINE | ID: mdl-30207401

ABSTRACT

ABSTRACT

A distance- and orientation-factor-dependent FRET system is a useful and attractive approach to the investigation of the conformational dynamics of nucleosomes. In this study, the application of the highly emissive nucleobase th dG-tC FRET pair to 601ânucleosomes is reported. It was found that the th dG-tC FRET pair was successfully incorporated to 145âbp 601âsequences, and different FRET efficiencies were obtained for the designated donor and acceptor positions in the nucleosome.

Assuntos

Desoxiguanosina/química; Transferência Ressonante de Energia de Fluorescência; Nucleossomos/química; Fenotiazinas/química; Pareamento de Bases; DNA/química; DNA/metabolismo; Desoxiguanosina/metabolismo; Modelos Moleculares; Nucleossomos/metabolismo; Fenotiazinas/metabolismo

Palavras-chave

DNA; FRET pair thdG-tC; genetics; nucleobase analogues; nucleosomes

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fenotiazinas / Nucleossomos / Transferência Ressonante de Energia de Fluorescência / Desoxiguanosina Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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