Tetrode Recording from the Hippocampus of Behaving Mice Coupled with Four-Point-Irradiation Closed-Loop Optogenetics: A Technique to Study the Contribution of Hippocampal SWR Events to Learning.
eNeuro
; 5(4)2018.
Article
em En
| MEDLINE
| ID: mdl-30225344
ABSTRACT
With the advent of optogenetics, it became possible to change the activity of a targeted population of neurons in a temporally controlled manner. To combine the advantages of 60-channel in vivo tetrode recording and laser-based optogenetics, we have developed a closed-loop recording system that allows for the actual electrophysiological signal to be used as a trigger for the laser light mediating the optogenetic intervention. We have optimized the weight, size, and shape of the corresponding implant to make it compatible with the size, force, and movements of a behaving mouse, and we have shown that the system can efficiently block sharp wave ripple (SWR) events using those events themselves as a trigger. To demonstrate the full potential of the optogenetic recording system we present a pilot study addressing the contribution of SWR events to learning in a complex behavioral task.
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Base de dados:
MEDLINE
Assunto principal:
Comportamento Animal
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Eletroencefalografia
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Ondas Encefálicas
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Optogenética
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Hipocampo
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Aprendizagem
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article