A tissue-specific self-interacting chromatin domain forms independently of enhancer-promoter interactions.
Nat Commun
; 9(1): 3849, 2018 09 21.
Article
em En
| MEDLINE
| ID: mdl-30242161
ABSTRACT
Self-interacting chromatin domains encompass genes and their cis-regulatory elements; however, the three-dimensional form a domain takes, whether this relies on enhancer-promoter interactions, and the processes necessary to mediate the formation and maintenance of such domains, remain unclear. To examine these questions, here we use a combination of high-resolution chromosome conformation capture, a non-denaturing form of fluorescence in situ hybridisation and super-resolution imaging to study a 70 kb domain encompassing the mouse α-globin regulatory locus. We show that this region forms an erythroid-specific, decompacted, self-interacting domain, delimited by frequently apposed CTCF/cohesin binding sites early in terminal erythroid differentiation, and does not require transcriptional elongation for maintenance of the domain structure. Formation of this domain does not rely on interactions between the α-globin genes and their major enhancers, suggesting a transcription-independent mechanism for establishment of the domain. However, absence of the major enhancers does alter internal domain interactions. Formation of a loop domain therefore appears to be a mechanistic process that occurs irrespective of the specific interactions within.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Sequências Reguladoras de Ácido Nucleico
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article