The electronic and magnetic properties of rubredoxin: a low-temperature magnetic circular dichroism study.
Biochim Biophys Acta
; 911(1): 71-80, 1987 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-3024732
ABSTRACT
Oxidized rubredoxin from Clostridium pasteurianum has been investigated by magnetic circular dichroism (MCD) spectroscopy over the temperature range 1.5 to 150 K and at magnetic fields between 0 and 4.5 tesla. The results show that studies of the temperature and field dependence of MCD transitions afford insight into the polarization of electronic transitions for ground states with large g-value anisotropy, in addition to estimates of ground-state g values and zero-field splitting parameters. In agreement with the assignment made by Eaton and Lovenberg (Eaton, W.A. and Lovenberg, W. (1973) in Iron-Sulfur Proteins, Vol. II (Lovenberg, W., ed.), pp. 131-162, Academic Press, New York), the ultraviolet-visible spectrum of oxidized rubredoxin is assigned to two S----Fe(III) charge transfer transitions (both 6A1----6T2 under tetrahedral symmetry), each spanning a range of 650-430 nm and 430-330 nm, respectively. The observed splitting in each of these transitions is attributed to a predominant axial distortion in the excited state resulting in effective D2d symmetry.
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Base de dados:
MEDLINE
Assunto principal:
Rubredoxinas
/
Ferredoxinas
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article