Engineering a responsive DNA triple helix into an octahedral DNA nanostructure for a reversible opening/closing switching mechanism: a computational and experimental integrated study.
Nucleic Acids Res
; 46(19): 9951-9959, 2018 11 02.
Article
em En
| MEDLINE
| ID: mdl-30247614
ABSTRACT
We propose an experimental and simulative approach to study the effect of integrating a DNA functional device into a large-sized DNA nanostructure. We selected, as a test bed, a well-known and characterized pH-dependent clamp-switch, based on a parallel DNA triple helix, to be integrated into a truncated octahedral scaffold. We designed, simulated and experimentally characterized two different functionalized DNA nanostructures, with and without the presence of a spacer between the scaffold and the functional elements. The experimental and simulative data agree in validating the need of a spacer for the occurrence of the pH dependent switching mechanism. The system is fully reversible and the switching can be monitored several times without any perturbation, maintaining the same properties of the isolated clamp switch in solution.
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1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Nanoestruturas
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Nanocápsulas
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Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article