A Photoactivatable BODIPY Probe for Localization-Based Super-Resolution Cellular Imaging.
Angew Chem Int Ed Engl
; 57(39): 12685-12689, 2018 09 24.
Article
em En
| MEDLINE
| ID: mdl-30247796
ABSTRACT
The synthesis and application of a photoactivatable boron-alkylated BODIPY probe for localization-based super-resolution microscopy is reported. Photoactivation and excitation of the probe is achieved by a previously unknown boron-photodealkylation reaction with a single low-power visible laser and without requiring the addition of reducing agents or oxygen scavengers in the imaging buffer. These features lead to a versatile probe for localization-based microscopy of biological systems. The probe can be easily linked to nucleophile-containing molecules to target specific cellular organelles. By attaching paclitaxel to the photoactivatable BODIPY, inâ
vitro and inâ
vivo super-resolution imaging of microtubules is demonstrated. This is the first example of single-molecule localization-based super-resolution microscopy using a visible-light-activated BODIPY compound as a fluorescent probe.
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Base de dados:
MEDLINE
Assunto principal:
Compostos de Boro
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Luz
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article