Reversibility of gelsolin/actin interaction in macrophages. Evidence of Ca2+-dependent and Ca2+-independent pathways.
J Exp Med
; 165(1): 97-106, 1987 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-3025333
ABSTRACT
We have developed an immunoadsorption technique for quantitating EGTA-resistant gelsolin/actin complexes in macrophages extracted with Triton X-100. We report here that the proportion of gelsolin complexed irreversibly to actin is low in freshly harvested macrophages. The amount of the EGTA-resistant complex increases spontaneously during incubation of the cells in suspension at 37 degrees C, or after exposure to the Ca2+ ionophore ionomycin. On the other hand, exposure of suspended cells to the chemotactic oligopeptide, FMLP, or plating of the cells onto tissue culture dishes causes the EGTA-resistant complex to dissociate rapidly. Plating even prevents Ca2+ ionomycin-treated cells with elevated intracellular Ca2+ from inducing this complex. Therefore, our results suggest that macrophages possess a mechanism, not directly involving Ca2+, for dissociating actin/gelsolin EGTA-resistant complexes. This mechanism may be a Ca2+-independent signal for leukocyte activation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto
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Proteínas de Ligação ao Cálcio
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Cálcio
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Actinas
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Macrófagos
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Proteínas dos Microfilamentos
Limite:
Animals
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article