Mitochondrial dysfunction in fibroblasts of Multiple System Atrophy.
Biochim Biophys Acta Mol Basis Dis
; 1864(12): 3588-3597, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30254015
ABSTRACT
Multiple System Atrophy is a severe neurodegenerative disorder which is characterized by a variable clinical presentation and a broad neuropathological spectrum. The pathogenic mechanisms are almost completely unknown. In the present study, we established a cellular model of MSA by using fibroblasts' primary cultures and performed several experiments to investigate the causative mechanisms of the disease, with a particular focus on mitochondrial functioning. Fibroblasts' analyses (7 MSA-P, 7 MSA-C and 6 healthy controls) displayed several anomalies in patients an impairment of respiratory chain activity, in particular for succinate Coenzyme Q reductase (pâ¯<â¯0.05), and a reduction of complex II steady-state level (pâ¯<â¯0.01); a reduction of Coenzyme Q10 level (pâ¯<â¯0.001) and an up-regulation of some CoQ10 biosynthesis enzymes, namely COQ5 and COQ7; an impairment of mitophagy, demonstrated by a decreased reduction of mitochondrial markers after mitochondrial inner membrane depolarization (pâ¯<â¯0.05); a reduced basal autophagic activity, shown by a decreased level of LC3 II (pâ¯<â¯0.05); an increased mitochondrial mass in MSA-C, demonstrated by higher TOMM20 levels (pâ¯<â¯0.05) and suggested by a wide analysis of mitochondrial DNA content in blood of large cohorts of patients. The present study contributes to understand the causative mechanisms of Multiple System Atrophy. In particular, the observed impairment of respiratory chain activity, mitophagy and Coenzyme Q10 biosynthesis suggests that mitochondrial dysfunction plays a crucial role in the pathogenesis of the disease. Furthermore, these findings will hopefully contribute to identify novel therapeutic targets for this still incurable disorder.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Atrofia de Múltiplos Sistemas
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Fibroblastos
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Mitocôndrias
Tipo de estudo:
Prognostic_studies
Limite:
Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article