Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation.
Curr Microbiol
; 75(12): 1649-1654, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30267141
ABSTRACT
Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.
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Base de dados:
MEDLINE
Assunto principal:
Esparteína
/
Proteínas de Bactérias
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Esteroide 17-alfa-Hidroxilase
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Pseudomonas putida
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Genoma Bacteriano
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article