Micro Imaging Displays the Sucrose Landscape within and along Its Allocation Pathways.
Plant Physiol
; 178(4): 1448-1460, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30275056
ABSTRACT
Sucrose (Suc) is the major transport sugar in plants and plays a primary role as an energy source and signal in adaptive and stress responses. An ability to quantify Suc over time and space would serve to advance our understanding of these important processes. Current technologies used for Suc mapping are unable to quantitatively visualize its distribution within tissues. Here, we present an infrared-based microspectroscopic method that allows for the quantitative visualization of Suc at a microscopic level of resolution (â¼12 µm). This method can successfully model the sugar concentration in individual vascular bundles and within a complex organ such as the stem, leaf, or seed. The sensitivity of the assay ranges from 20 to 1,000 mm We applied this method to the cereal crop barley (Hordeum vulgare) and the model plant Arabidopsis (Arabidopsis thaliana) to highlight the potential of the procedure for resolving the spatial distribution of metabolites. We also discuss the relevance of the method for studies on carbon allocation and storage in the context of crop improvement.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sacarose
/
Hordeum
/
Arabidopsis
/
Espectroscopia de Infravermelho com Transformada de Fourier
/
Imagem Molecular
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article