Patterns of mutation within an emerging endemic lineage of HEV-3a.
J Viral Hepat
; 26(1): 191-198, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30315669
ABSTRACT
The hepatitis E virus can cause chronic infections in immuno-suppressed patients, and cases have been on the rise globally. Viral mutations during such infections are difficult to characterize. We deep-sequenced viral populations from 15 immunocompromised patients with chronic HEV to identify the viral lineage and describe viral mutational hotspots within and across patients. A total of 21 viral RNA samples were collected between 2012 and 2017 from a single hospital in Singapore. Sequences covering a total of 3894 bp of the HEV genome were obtained. Phylogenetic analyses identified all sequences as belonging to the HEV-3a sub-clade and clearly indicate a unique local lineage. Deep sequencing reveals variable viral population complexity during infections. Comparisons of viral samples from the same patients spaced 2-19 months apart revealed rapid nucleotide replacements in the dominant viral sequence in both ribavirin treated and treatment-naive patients. Mutational hotspots were identified within ORF3 and the PCP/HVR domain of ORF1.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
RNA Viral
/
Vírus da Hepatite E
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Hepatite E
/
Mutação
Tipo de estudo:
Prognostic_studies
Limite:
Humans
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article