Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles.
Biochem Biophys Res Commun
; 505(4): 1097-1102, 2018 11 10.
Article
em En
| MEDLINE
| ID: mdl-30316514
ABSTRACT
The delivery of mRNA is advantageous over DNA delivery as it is transient and does not carry the risk of genomic DNA integration. However, there are currently few efficient mRNA delivery options available, especially for hard-to-transfect cell types, and thus new delivery methods are needed. To this end, we have established a novel mRNA delivery system utilizing chimeric virus-like particles (VLPs). We generated a novel VLP by fusing protein G of Vesicular stomatitis virus (VSV-G) with a ribosomal protein L7Ae of Archeoglobus fulgidus. This system allowed the efficient delivery of EGFP mRNA which was independent from the presence of BoxC/D motif in the mRNA sequence. Our VSVG-L7Ae VLP system demonstrated high transduction efficacy in hard-to-transfect cell lines, such as human induced pluripotent stem cells (iPS cells) and monocytes. In summary, this platform may serve as an efficient and transient transgene delivery tool for an mRNA of interest.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
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RNA Mensageiro
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Glicoproteínas de Membrana
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Proteínas do Envelope Viral
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Técnicas de Transferência de Genes
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article