Association mapping, transcriptomics, and transient expression identify candidate genes mediating plant-pathogen interactions in a tree.
Proc Natl Acad Sci U S A
; 115(45): 11573-11578, 2018 11 06.
Article
em En
| MEDLINE
| ID: mdl-30337484
ABSTRACT
Invasive microbes causing diseases such as sudden oak death negatively affect ecosystems and economies around the world. The deployment of resistant genotypes for combating introduced diseases typically relies on breeding programs that can take decades to complete. To demonstrate how this process can be accelerated, we employed a genome-wide association mapping of ca 1,000 resequenced Populus trichocarpa trees individually challenged with Sphaerulina musiva, an invasive fungal pathogen. Among significant associations, three loci associated with resistance were identified and predicted to encode one putative membrane-bound L-type receptor-like kinase and two receptor-like proteins. A susceptibility-associated locus was predicted to encode a putative G-type D-mannose-binding receptor-like kinase. Multiple lines of evidence, including allele analysis, transcriptomics, binding assays, and overexpression, support the hypothesized function of these candidate genes in the P. trichocarpa response to S. musiva.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Proteínas de Plantas
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Regulação da Expressão Gênica de Plantas
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Populus
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Saccharomycetales
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Interações Hospedeiro-Patógeno
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Transcriptoma
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article