Detection and Functional Analysis of TP53 Mutations in CLL.
Methods Mol Biol
; 1881: 63-81, 2019.
Article
em En
| MEDLINE
| ID: mdl-30350198
ABSTRACT
Chronic lymphocytic leukemia (CLL) represents a prototype disease in which TP53 gene defects lead to inferior prognosis. Here, we present two distinct methodologies which can be used to identify TP53 mutations in CLL patients; both protocols are primarily intended for research purposes. The functional analysis of separated alleles in yeast (FASAY) can be flexibly adapted to a variable number of samples and provides an immediate functional readout of identified mutations. Amplicon-based next-generation sequencing then allows for a high throughput and accurately detects subclonal TP53 variants (sensitivity <1% of mutated cells).
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Leucemia Linfocítica Crônica de Células B
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Proteína Supressora de Tumor p53
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Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article