Derivation of clones close to met by preparative field inversion gel electrophoresis.
Science
; 236(4806): 1305-8, 1987 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-3035716
ABSTRACT
The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used to selectively isolate clones from the environment of genetically linked markers, and to select a subset of these clones containing sequences next to specific restriction sites rare in mammalian DNA. This approach has been used to generate a library highly enriched in sequences closely linked to the cystic fibrosis marker met. One clone derived from the end of a Not I restriction fragment containing the met sequence was analyzed in detail and localized within a long range map to a position 300 kilobase pairs 5' of the metD sequence.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Marcadores Genéticos
/
Mapeamento Cromossômico
/
Fibrose Cística
/
Eletroforese
Limite:
Humans
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article