Downregulation of microRNA30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients.
Mol Med Rep
; 18(6): 5799-5806, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30365083
ABSTRACT
MicroRNAs (miRs) are short, highly conserved small noncoding RNA molecules with fundamental roles in regulating gene expression. To identify miR biomarkers associated with idiopathic pulmonary fibrosis (IPF), the expression pattern of miRs in exosomes from bronchoalveolar lavage fluid (BALF) of elderly patients with IPF were evaluated. Highthroughput quantitative detection of miR expression using a microarray indicated that miR125b, miR128, miR21, miR100, miR1403p and miR374b were upregulated in patients with IPF, while let7d, miR103, miR26 and miR30a5p were downregulated. The expression level of miR30a5p was further examined, and its potential target genes were predicted using target gene prediction analysis software. A direct regulatory association was confirmed between miR30a5p and TGFß activated kinase 1/MAP3K7 binding protein 3 (TAB3) via a dualluciferase reporter assay. Overexpression of miR30a5p decreased TAB3, αsmooth muscle actin and fibronectin expression in A549 cells with or without transforming growth factorß1 treatment. The decreased expression of miR30a in the BALF of patients with IPF, along with the consequential increase in TAB3 expression, may be a crucial factor in IPF progression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Líquido da Lavagem Broncoalveolar
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Regulação da Expressão Gênica
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MicroRNAs
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Fibrose Pulmonar Idiopática
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MicroRNA Circulante
Tipo de estudo:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article