Recognition protein C1q of innate immunity agglutinates nanodiamonds without activating complement.
Nanomedicine
; 18: 292-302, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-30368000
ABSTRACT
Nanodiamonds are promising nanomedicines for diagnostic and therapeutic applications. As nanodiamonds are mainly administered intravenously, it is critical to understand the humoral immune response upon exposure to nanodiamonds. Here, we report the interactions of pristine, oxidized, and PEG-functionalized nanodiamonds with human complement, an important part of our humoral innate immunity. In particular, we report the nanodiamond binding properties of the recognition protein of the classical complement pathway C1q, which also takes part in many other physiological and pathological processes. Our results show similar trends in the effects of C1q on the three types of nanodiamonds. Complement activation assays using human serum show that the nanodiamonds trigger slight activities via the alternative pathway and no response via the classical pathway. Nevertheless, surface plasmon resonance shows that C1q binds the nanodiamonds and transmission electron microscopy reveals their agglutination. Studies with macrophages further show that C1q attachment affects their phagocytosis and cytokine response.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complemento C1q
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Ativação do Complemento
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Nanodiamantes
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Imunidade Inata
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article