Enzyme-linked immunosorbent assay for the detection of antibodies to bovine virus diarrhea virus in sera from border disease virus-infected sheep.
Can J Vet Res
; 51(2): 281-3, 1987 Apr.
Article
em En
| MEDLINE
| ID: mdl-3038292
ABSTRACT
An enzyme-linked immunosorbent assay (ELISA) was established for the rapid detection of specific antibodies against the causative agent of border disease in ovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified bovine virus diarrhea virus was used as test antigen. The optimal amount of antigen was 0.5 microgram/well, and the optimal concentration of conjugate was at 1/4,000 dilution. A total of 20 ovine serum samples, which had been collected from animals with or without border disease, were compared by ELISA and serum neutralization test for the detection of border disease-specific antibodies. ELISA was shown to be equally specific but less time-consuming and easier to perform than serum neutralization test. A positive correlation (r = 0.60) between the two tests was found.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Ovinos
/
Doença da Fronteira
/
Ensaio de Imunoadsorção Enzimática
/
Pestivirus
/
Vírus da Diarreia Viral Bovina
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article