Targeting CD40 enhances antibody- and CD8-mediated protection against respiratory syncytial virus infection.
Sci Rep
; 8(1): 16648, 2018 11 09.
Article
em En
| MEDLINE
| ID: mdl-30413743
ABSTRACT
Respiratory Syncytial Virus (RSV) infects almost all children under the age of one and is the leading cause of hospitalization among infants. Despite several decades of research with dozens of candidate vaccines being vigorously evaluated in pre-clinical and clinical studies, there is no licensed vaccine available to date. Here, the RSV fusion protein (F) was fused with CD40 ligand and delivered by an adenoviral vector into BALB/c mice where the CD40 ligand serves two vital functions as a molecular adjuvant and an antigen-targeting molecule. In contrast to a formaldehyde-inactivated vaccine, the vectored vaccine effectively protected animals against RSV without inducing enhanced respiratory disease. This protection involved a robust induction of neutralizing antibodies and memory CD8 T cells, which were not observed in the inactivated vaccine group. Finally, the vectored vaccine was able to elicit long-lasting protection against RSV, one of the most challenging issues in RSV vaccine development. Further studies indicate that the long lasting protection elicited by the CD40 ligand targeted vaccine was mediated by increased levels of effector memory CD8 T cell 3 months post-vaccination.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus Sinciciais Respiratórios
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Proteínas Virais de Fusão
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Infecções por Vírus Respiratório Sincicial
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Linfócitos T CD8-Positivos
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Ligante de CD40
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Vacinas contra Vírus Sincicial Respiratório
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Anticorpos Antivirais
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article