Purification of a conjugated polysaccharide vaccine using tangential flow diafiltration.
Biotechnol Bioeng
; 116(3): 591-597, 2019 03.
Article
em En
| MEDLINE
| ID: mdl-30450582
ABSTRACT
Conjugated vaccines prepared from the capsular polysaccharide of Streptococcus pneumoniae can provide immunization against invasive pneumococcal disease, meningitis, and otitis media. One of the critical steps in the production of these vaccines is the removal of free (unreacted) polysaccharides from the protein-polysaccharide conjugate. Experimental studies were performed to evaluate the effects of membrane pore size, filtrate flux, and solution conditions on the transmission of both the conjugate and free polysaccharide through different ultrafiltration membranes. Conjugate purification was done using diafiltration performed in a linearly-scalable tangential flow filtration cassette. More than 98% of the free polysaccharide was removed within a 5-diavolume diafiltration process, which is a significant improvement over previously reported results for purification of similar conjugated vaccines. These results clearly demonstrate the opportunities for using ultrafiltration/diafiltration for the final purification of conjugated vaccine products.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
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Vacinas Bacterianas
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Ultrafiltração
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Cápsulas Bacterianas
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Vacinas Conjugadas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article