Changes of Cell Biochemical States Are Revealed in Protein Homomeric Complex Dynamics.
Cell
; 175(5): 1418-1429.e9, 2018 11 15.
Article
em En
| MEDLINE
| ID: mdl-30454649
ABSTRACT
We report here a simple and global strategy to map out gene functions and target pathways of drugs, toxins, or other small molecules based on "homomer dynamics" protein-fragment complementation assays (hdPCA). hdPCA measures changes in self-association (homomerization) of over 3,500 yeast proteins in yeast grown under different conditions. hdPCA complements genetic interaction measurements while eliminating the confounding effects of gene ablation. We demonstrate that hdPCA accurately predicts the effects of two longevity and health span-affecting drugs, the immunosuppressant rapamycin and the type 2 diabetes drug metformin, on cellular pathways. We also discovered an unsuspected global cellular response to metformin that resembles iron deficiency and includes a change in protein-bound iron levels. This discovery opens a new avenue to investigate molecular mechanisms for the prevention or treatment of diabetes, cancers, and other chronic diseases of aging.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Sirolimo
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Ferro
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Metaloproteínas
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Metformina
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article