A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms.

Banos, Stefanos; Lentendu, Guillaume; Kopf, Anna; Wubet, Tesfaye; Glöckner, Frank Oliver; Reich, Marlis

Banos, Stefanos; Lentendu, Guillaume; Kopf, Anna; Wubet, Tesfaye; Glöckner, Frank Oliver; Reich, Marlis.

Afiliação

Banos S; Molecular Ecology, Institute of Ecology, FB02, University of Bremen, Leobener Str. 2, 28359, Bremen, Germany.
Lentendu G; Department of Soil Ecology, Helmholtz Centre for Environmental Research GmbH - UFZ, Halle-Saale, Germany.
Kopf A; Department of Ecology, University of Kaiserslautern, Kaiserslautern, Germany.
Wubet T; Microbial Genomics and Bioinformatics Research Group, Max Planck Institute for Marine Microbiology, Bremen, Germany.
Glöckner FO; Department of Soil Ecology, Helmholtz Centre for Environmental Research GmbH - UFZ, Halle-Saale, Germany.
Reich M; Present address: Department of Community Ecology, Helmholtz Centre for Environmental Research GmbH - UFZ, Halle-Saale, Germany.

BMC Microbiol ; 18(1): 190, 2018 11 20.

Article em En | MEDLINE | ID: mdl-30458701

Assuntos

Primers do DNA/genética; DNA Fúngico/genética; RNA Ribossômico 18S/genética; Biodiversidade; Fungos/classificação; Fungos/genética; Fungos/isolamento & purificação; Sequenciamento de Nucleotídeos em Larga Escala; Reação em Cadeia da Polimerase; Especificidade da Espécie

Palavras-chave

18S rRNA gene sequence (SSU) primer; Annealing blocking oligonucleotides; Co-amplification; Community survey; FF390; FR-1; Fungal biodiversity; Fungi; Real-time Q-PCR; Taxonomic classification

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA Fúngico / RNA Ribossômico 18S / Primers do DNA Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google