Interlaboratory Validation of a Method for Hepatitis E Virus RNA Detection in Meat and Meat Products.
Food Environ Virol
; 11(1): 1-8, 2019 03.
Article
em En
| MEDLINE
| ID: mdl-30465253
ABSTRACT
Increasing numbers of hepatitis E cases are currently recognized in many European countries. The zoonotic hepatitis E virus (HEV) genotype 3 mainly circulates in domestic pigs and wild boars, and can be transmitted to humans via consumption of insufficiently heated meat or meat products produced from those animals. Here, a detailed protocol for detection of HEV RNA in meat products is provided, which is based on the method originally described by Szabo et al. (Intl J Food Microbiol 215149-156, 2015). It consists of a TRI Reagent®/chloroform-based food matrix homogenization, a silica bead-based RNA extraction and a real-time RT-PCR-based RNA detection. The method was further validated in a ring trial with nine independent laboratories using pork liver sausage samples artificially contaminated with different amounts of HEV. The results indicate sufficient sensitivity, specificity, and accuracy of the method for its broad future use in survey studies, routine food control or outbreak investigations.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Virologia
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RNA Viral
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Vírus da Hepatite E
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Técnicas de Amplificação de Ácido Nucleico
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Carne
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article