Validation of the use of 32P-orthophosphate as a probe for monitoring DNA replication in pancreatic islet cells.
Diabetes Res
; 7(3): 141-4, 1988 Mar.
Article
em En
| MEDLINE
| ID: mdl-3046821
ABSTRACT
This study aims at validating the use of 32P-orthophosphate as a probe for monitoring DNA replication instead of [3H]-thymidine where the specific activity of the latter is affected by perturbation of the cellular environment. It was found that to obtain a clean DNA fraction for determining the incorporation of the label, protein must be removed by the use of Proteinase K, and RNA by alkaline hydrolysis. Alkaline hydrolysis of RNA was found superior to RNAse digestion in not leaving behind large RNA fragments that may contaminate the DNA fraction.
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Base de dados:
MEDLINE
Assunto principal:
Fosfatos
/
Ilhotas Pancreáticas
/
Replicação do DNA
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article