Strain-specific effect on biphasic DNA binding by HIV-1 integrase.
AIDS
; 33(3): 588-592, 2019 03 01.
Article
em En
| MEDLINE
| ID: mdl-30475264
ABSTRACT
The oligomerization of HIV-1 integrase onto DNA is not well understood. Here we show that HIV-1 integrase binds the DNA in biphasic (high-affinity and low-affinity) modes. For HIV-1 subtype B, the high-affinity mode is â¼100-fold greater than the low-affinity mode (Kd.DNAâ=â37 and 3400ânmol/l, respectively). The Kd.DNA values of patient-derived integrases containing subtype-specific polymorphisms were affected two- to four-fold, suggesting that polymorphisms may have an influence on effective-concentrations of inhibitors, as these inhibitors preferably bind to integrase-DNA complex.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
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Infecções por HIV
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HIV-1
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Integração Viral
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Integrase de HIV
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article