Macrophage depletion with clodronate-containing liposomes affects the incidence and development of rheumatoid arthritis.
Z Rheumatol
; 78(10): 996-1003, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-30478637
ABSTRACT
OBJECTIVE:
This study investigated the effect of macrophage depletion with clodronate-containing liposomes (Clo-lip) on the incidence and development of rheumatoid arthritis (RA).METHODS:
The effect of macrophage depletion with Clo-lip in the spleen was assessed by HE (haematoxylin and eosin) staining and immunohistochemistry (IHC). Thirty BALB/c mice were randomly divided into three groups, which were administered PBS-lip, Clo-lip, or normal saline. RA model mice were then created and the appearance of the paws was observed. Expression of CD68 by macrophages was examined by immunofluorescence on the 49th day. Forty-five RA model mice were created and randomly divided into three groups. The experiment group was administered Clo-lip at different timepoints. The degree of arthritis score was recorded during the administration. Histological features were detected by HE staining on the 84th day.RESULTS:
Compared to controls, horseshoe-shaped nuclei and multi-core large cells were reduced in the experimental group (HE stain; pâ¯< 0.05). Brown tag-CD68 and tag-CD80 macrophages were fewer in the experimental group than in the control group (immunohistochemistry; pâ¯< 0.05). Furthermore, the degree of arthritis score in the experimental group was significantly decreased (pâ¯< 0.05). HE staining showed that there was no or less inflammatory cell infiltration in the articular cavity in mice in the experimental group, and that the percentage of CD68+ macrophage cells in synovial cells was significantly lower than in the control group (pâ¯< 0.05).CONCLUSION:
Macrophage depletion with Clo-lip can affect the incidence and development of RA.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Artrite Reumatoide
/
Ácido Clodrônico
/
Lipossomos
/
Macrófagos
Tipo de estudo:
Incidence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article