Your browser doesn't support javascript.
loading
Fibrin glue displays promising in vitro characteristics as a potential carrier of adipose progenitor cells for tissue regeneration.
Krug, Christian; Beer, Anita; Hartmann, Bastian; Prein, Carina; Clause-Schaumann, Hauke; Holzbach, Thomas; Aszodi, Attila; Giunta, Riccardo Enzo; Saller, Maximilian Michael; Volkmer, Elias.
Afiliação
  • Krug C; Clinic for Hand and Plastic Surgery, Spital Thurgau, Frauenfeld, Switzerland.
  • Beer A; Department of Hand, Plastic and Aesthetic Surgery, Munich University Hospital, Ludwig Maximilians University, Munich, Germany.
  • Hartmann B; Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Munich University Hospital, Ludwig Maximilians University, Munich, Germany.
  • Prein C; Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Munich University Hospital, Ludwig Maximilians University, Munich, Germany.
  • Clause-Schaumann H; Center for Applied Tissue Engineering and Regenerative Medicine (CANTER), Munich University of Applied Sciences, Munich, Germany.
  • Holzbach T; Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Munich University Hospital, Ludwig Maximilians University, Munich, Germany.
  • Aszodi A; Center for Applied Tissue Engineering and Regenerative Medicine (CANTER), Munich University of Applied Sciences, Munich, Germany.
  • Giunta RE; Center for Applied Tissue Engineering and Regenerative Medicine (CANTER), Munich University of Applied Sciences, Munich, Germany.
  • Saller MM; Clinic for Hand and Plastic Surgery, Spital Thurgau, Frauenfeld, Switzerland.
  • Volkmer E; Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Munich University Hospital, Ludwig Maximilians University, Munich, Germany.
J Tissue Eng Regen Med ; 13(3): 359-368, 2019 03.
Article em En | MEDLINE | ID: mdl-30515986
Adipose-derived multipotent stem/progenitor cells (ASPCs) were shown to be ideal candidates for cell-based regenerative therapies. Yet, despite their huge potential, successful clinical applications are still rare. It was suggested that the efficacy of ASPCs at the recipient site depends on the vehicle of cell delivery. In this study, for preparation of a murine critical-size nerve defect model, we assessed the commercially available fibrin gel (ARTISS) as a potential cell carrier. In a thorough in vitro analysis, we investigated cell-fibrin interactions and analyzed the distribution and the long-term behavior of ASPCs cultivated in fibrin gel under normoxic and hypoxic conditions. ASPCs attached to the surface of a thin fibrin layer (two-dimensional condition) and spread with the abundant formation of actin stress fibers. Cells cultured within a fibrin matrix (three-dimensional condition) displayed a uniform distribution and formed interconnected networks while exhibiting strong cell-matrix interactions. Using time-lapse analysis, cells were found to migrate out of the gel and subsequently proliferated robustly both under hypoxic and normoxic conditions. During 14 days of culture in fibrin gel, ASPCs showed high viability, metabolic, and remodeling activities. At the end of the culture period, the fibrin matrix was degraded entirely accompanied by an upregulation of matrix metalloproteinases. In conclusion, fibrin gel stands out as a valuable biomaterial for delivering vital and active cells to damaged tissues. As a direct proof, ASPCs carried in a fibrin matrix will be evaluated in a murine critically sized peripheral nerve repair model.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regeneração / Células-Tronco / Adesivo Tecidual de Fibrina / Tecido Adiposo Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regeneração / Células-Tronco / Adesivo Tecidual de Fibrina / Tecido Adiposo Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article