Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM.
Nat Plants
; 5(1): 14-17, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30531939
ABSTRACT
Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Arabidopsis
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Sistemas CRISPR-Cas
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Edição de Genes
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Proteína 9 Associada à CRISPR
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article