SLIM microscopy allows for visualization of DNA-containing liposomes designed for sperm-mediated gene transfer in cattle.

Rubessa, Marcello; Lotti, Samantha N; Kandel, Mikhail E; Popescu, Gabriel; Wheeler, Mathew B

Rubessa, Marcello; Lotti, Samantha N; Kandel, Mikhail E; Popescu, Gabriel; Wheeler, Mathew B.

Afiliação

Rubessa M; Department of Animal Sciences, University of Illinois, Urbana-Champaign, IL, 61801, USA.
Lotti SN; Department of Animal Sciences, University of Illinois, Urbana-Champaign, IL, 61801, USA.
Kandel ME; Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute of Advanced Science and Technology, Urbana, USA.
Popescu G; Department of Bioengineering, University of Illinois, Urbana, IL, 61801, USA.
Wheeler MB; Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute of Advanced Science and Technology, Urbana, USA.

Mol Biol Rep ; 46(1): 695-703, 2019 Feb.

Article em En | MEDLINE | ID: mdl-30539382

Assuntos

DNA/análise; Técnicas de Transferência de Genes; Microscopia/métodos; Espermatozoides/metabolismo; Animais; Bovinos; Proteínas de Fluorescência Verde/metabolismo; Lipossomos; Masculino; Plasmídeos/metabolismo

Palavras-chave

GFP; Liposome; Microscopy; SLIM; Sperm; Sperm-mediated gene transfer

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatozoides / DNA / Técnicas de Transferência de Genes / Microscopia Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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