Antibody-Linked Fluorogen-Activating Proteins for Antigen Detection and Cell Ablation.
Bioconjug Chem
; 30(1): 63-69, 2019 01 16.
Article
em En
| MEDLINE
| ID: mdl-30543409
ABSTRACT
We demonstrate selective labeling of cell surface proteins using fluorogen-activating proteins (FAPs) conjugated to standard immunoglobulins (IgGs). Conjugation was achieved with a polypeptide reagent comprised of an N-terminal photoactivatable Fc-binding domain and a C-terminal FAP domain. The resulting FAP-antibody conjugates were effective agents for protein detection and cell ablation in cultured mammalian cells and for visualizing cell-cell contacts using a tethered fluorogen assay. Because our approach allows FAP-antibody conjugates to be generated for most currently available IgGs, it should have broad utility for experimental and therapeutic applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Corantes Fluorescentes
/
Cetuximab
/
Trastuzumab
/
Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article