LncRNA-H19 activates CDC42/PAK1 pathway to promote cell proliferation, migration and invasion by targeting miR-15b in hepatocellular carcinoma.
Genomics
; 111(6): 1862-1872, 2019 12.
Article
em En
| MEDLINE
| ID: mdl-30543848
ABSTRACT
BACKGROUND:
Hepatocellular carcinoma (HCC) is one of the main causes of cancer-related death. This study aims to explore the role and underlying mechanism of H19 in HCC.METHODS:
qRT-PCR detected miR-15b-5p and H19 expression, as well as the mRNA level of EMT-associated genes. Western blotting detected protein level of EMT-associated genes. Immunohistochemistry (IHC) examined CDC42 in HCC tissues. Dual luciferase reporter assay verified the regulatory mechanism among H19, miR-15b and CDC42. Colony formation, wound healing assay, transwell, flow cytometry measured proliferation, migration, invasion and apoptosis, respectively.RESULTS:
H19 and CDC42 were up-regulated while miR-15b was down-regulated in HCC cells and tissues. miR-15b interacted with H19 and CDC42 3'-UTR. H19 knockdown inhibited proliferation, migration and invasion, and increased apoptosis, which was rescued by miR-15b inhibitor. H19 knockdown suppressed CDC42/PAK1 pathway and EMT progress.CONCLUSION:
H19 knockdown inhibited proliferation, migration and invasion, and promoted apoptosis of HCC cells via targeting miR-15b/CDC42/PAK1 axis.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Neoplásico
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Movimento Celular
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Carcinoma Hepatocelular
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Proteína cdc42 de Ligação ao GTP
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MicroRNAs
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Proliferação de Células
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Quinases Ativadas por p21
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RNA Longo não Codificante
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Neoplasias Hepáticas
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Proteínas de Neoplasias
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article