Aquaporin-4-dependent glymphatic solute transport in the rodent brain.
Elife
; 72018 12 18.
Article
em En
| MEDLINE
| ID: mdl-30561329
ABSTRACT
The glymphatic system is a brain-wide clearance pathway; its impairment contributes to the accumulation of amyloid-ß. Influx of cerebrospinal fluid (CSF) depends upon the expression and perivascular localization of the astroglial water channel aquaporin-4 (AQP4). Prompted by a recent failure to find an effect of Aqp4 knock-out (KO) on CSF and interstitial fluid (ISF) tracer transport, five groups re-examined the importance of AQP4 in glymphatic transport. We concur that CSF influx is higher in wild-type mice than in four different Aqp4 KO lines and in one line that lacks perivascular AQP4 (Snta1 KO). Meta-analysis of all studies demonstrated a significant decrease in tracer transport in KO mice and rats compared to controls. Meta-regression indicated that anesthesia, age, and tracer delivery explain the opposing results. We also report that intrastriatal injections suppress glymphatic function. This validates the role of AQP4 and shows that glymphatic studies must avoid the use of invasive procedures.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Astrócitos
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Aquaporina 4
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Sistema Glinfático
Tipo de estudo:
Systematic_reviews
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article