Investigation of the batch-to-batch inconsistencies of Collagen in PCL-Collagen nanofibers.

ABSTRACT

The application of tissue engineered constructs is an approach in regenerative therapies to support regeneration of damaged muscular tissue. Therefore the development of highly aligned electrospun scaffolds based on polycaprolactone (PCL) and collagen enables a versatile development towards tailor made applications. However, the application of natural polymers like collagen brings the risk of batch-to-batch inconsistencies, which influence the reproducibility of the electrospinning process. Aligned PCL-Collagen nanofibers were fabricated via electrospinning using benign solvents. The spinnability of different collagen batches and polymer concentrations in diluted acetic acid as solvent was investigated. Furthermore spinning parameters and fiber morphology were investigated in order to determine the most stable spinning conditions and analyze the batch-to-batch variations. Finally the effect of the solution temperature and the time of pure collagen in solution were investigated, to complete the analysis of the influences on the spinning behavior.