High-risk human papillomavirus detection in oropharyngeal cancers: Comparison of saliva sampling methods.
Head Neck
; 41(5): 1484-1489, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-30582230
ABSTRACT
BACKGROUND:
Accumulating evidence has suggested the utility of salivary oral rinse as a diagnostic fluid to detect oral human papillomavirus (HPV) DNA, but there are many methods for collecting saliva.METHODS:
Salivary oral rinse and unstimulated whole mouth saliva samples were collected from 45 oropharyngeal cancer (OPC) patients.RESULTS:
We show a positive correlation of HPV-16 E2 (r = 0.95, P < 0.0001) and E6/7 (r = 0.93, P < 0.0001) relative copy number as well as HPV genotypes in both sample methods. There was a significant correlation between the two sample methods in the ratio of HPV16 E2 to E6/7 DNA (r = 0.46, P < 0.01). Consistent with previous studies, a mixed HPV-16 form (episomal and integrated) was commonly found in both saliva and tumor samples.CONCLUSION:
Detection of HPV in saliva samples collected by either method yielded comparable results, and showed good sensitivity for detection of HPV derived from OPC.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Neoplasias Orofaríngeas
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Infecções por Papillomavirus
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Papillomavirus Humano 16
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
/
Middle aged
País como assunto:
Oceania
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article