Regulation of Plasma Membrane Nanodomains of the Water Channel Aquaporin-3 Revealed by Fixed and Live Photoactivated Localization Microscopy.
Nano Lett
; 19(2): 699-707, 2019 02 13.
Article
em En
| MEDLINE
| ID: mdl-30584808
ABSTRACT
Several aquaporin (AQP) water channels are short-term regulated by the messenger cyclic adenosine monophosphate (cAMP), including AQP3. Bulk measurements show that cAMP can change diffusive properties of AQP3; however, it remains unknown how elevated cAMP affects AQP3 organization at the nanoscale. Here we analyzed AQP3 nano-organization following cAMP stimulation using photoactivated localization microscopy (PALM) of fixed cells combined with pair correlation analysis. Moreover, in live cells, we combined PALM acquisitions of single fluorophores with single-particle tracking (spt-PALM). These analyses revealed that AQP3 tends to cluster and that the diffusive mobility is confined to nanodomains with radii of â¼150 nm. This domain size increases by â¼30% upon elevation of cAMP, which, however, is not accompanied by a significant increase in the confined diffusion coefficient. This regulation of AQP3 organization at the nanoscale may be important for understanding the mechanisms of water AQP3-mediated water transport across plasma membranes.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
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AMP Cíclico
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Células Epiteliais
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Aquaporina 3
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article