Comparative Transcriptome Analysis of the Skin-Specific Accumulation of Anthocyanins in Black Peanut ( Arachis hypogaea L.).
J Agric Food Chem
; 67(4): 1312-1324, 2019 Jan 30.
Article
em En
| MEDLINE
| ID: mdl-30614699
ABSTRACT
As an oil crop with good taste and profuse nutrition, peanut ( Arachis hypogaea L.) is grown worldwide, mainly for edible seeds. Black peanuts attract more attention for their appealing color and health-promoting anthocyanins. Here, two cyanidin-based anthocyanins and four quercetin-based flavonols were separated and identified from skins of two black cultivars (Zi Yu and Zi Guan) by HPLC-ESI-Q-TOF-MS. To study the anthocyanin accumulation, libraries constructed from the mRNA of skins of Zi Yu and white cultivar (Bai Yu) were sequenced, and 4042 differentially expressed genes were identified. Gene ontology and KEGG pathway analysis underlined the importance of the high expression of flavonoid biosynthetic and regulatory genes in seed skin of Zi Yu. Furthermore, expression profiles of these genes were analyzed carefully in four representative peanut cultivars. Altogether, these results strongly indicate that the up-regulation of transcriptional activators (AhMYB1, AhMYB2, and AhTT8) accounts for the skin-specific accumulation of anthocyanins in black peanut.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Arachis
/
Proteínas de Plantas
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Transcriptoma
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Antocianinas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article