Identification of calnexin as a diacylglycerol acyltransferase-2 interacting protein.
PLoS One
; 14(1): e0210396, 2019.
Article
em En
| MEDLINE
| ID: mdl-30615684
ABSTRACT
Triacylglycerol synthesis is catalyzed by acyl CoAdiacylglycerol acyltransferase-2 (DGAT2). DGAT2 is an integral membrane protein that is localized to the endoplasmic reticulum and interacts with lipid droplets. Using BioId, a method to detect proximal and interacting proteins, we identified calnexin as a DGAT2-interacting protein. Co-immunoprecipitation and proximity ligation assays confirmed this finding. We found that calnexin-deficient mouse embryonic fibroblasts had reduced intracellular triacylglycerol levels and fewer large lipid droplets (>1.0 µm2 area). Despite the alterations in triacylglycerol metabolism, in vitro DGAT2 activity, localization and protein stability were not affected by the absence of calnexin.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Calnexina
/
Retículo Endoplasmático
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Diacilglicerol O-Aciltransferase
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article