Legionella effector SetA as a general O-glucosyltransferase for eukaryotic proteins.
Nat Chem Biol
; 15(3): 213-216, 2019 03.
Article
em En
| MEDLINE
| ID: mdl-30617292
ABSTRACT
The identification of host protein substrates is key to understanding effector glycosyltransferases secreted by pathogenic bacteria and to using them for glycoprotein engineering. Here we report a chemical method for tagging, enrichment, and site-specific proteomic profiling of effector-modified proteins in host cells. Using this method, we discover that Legionella effector SetA α-O-glucosylates various eukaryotic proteins by recognizing a S/T-X-L-P/G sequence motif, which can be exploited to site-specifically introduce O-glucose on recombinant proteins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte de Monossacarídeos
/
Engenharia de Proteínas
/
Glicosiltransferases
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article