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miR­625­5p suppresses inflammatory responses by targeting AKT2 in human bronchial epithelial cells.
Qian, Fen-Hong; Deng, Xia; Zhuang, Qiong-Xin; Wei, Bin; Zheng, Dan-Dan.
Afiliação
  • Qian FH; Department of Respiratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China.
  • Deng X; Department of Respiratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China.
  • Zhuang QX; Department of Respiratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China.
  • Wei B; Department of Respiratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China.
  • Zheng DD; Department of Respiratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China.
Mol Med Rep ; 19(3): 1951-1957, 2019 Mar.
Article em En | MEDLINE | ID: mdl-30628701
Asthma is a common chronic inflammatory airway disease; however, whether microRNAs (miRs) could be used in the treatment of asthma remains unclear. The aim of the present study was to investigate the role of miR­625­5p in the inflammatory response of human bronchial epithelial cells (HBECs). Inflammation in the HBEC line, 16HBEC, was induced using different concentrations of lipopolysaccharide (LPS), which demonstrated that 1 µg/ml LPS was an appropriate concentration for further experiments. The association between protein kinase B2 (AKT2) and miR­625­5p was verified using a luciferase reporter assay. LPS was added to 16HBECs following the administration of miR­625­5p mimics or miR­625­5p inhibitors, and cells with silenced or overexpressed AKT2 levels. miR­625­5p was expressed at a high level in LPS­activated 16HBECs. Overexpression of miR­625­5p inhibited interleukin (IL)­6 and tumor necrosis factor (TNF)­α secretion in 16HBECs. Inhibition of miR­625­5p enhanced LPS­induced IL­6 and TNF­α secretion. miR­625­5p negatively regulated the expression of AKT2 in 16HBECs. A dual­luciferase reporter assay system confirmed that miR­625­5p directly targeted the 3'untranslated region of AKT2. Transfection with a small interfering RNA against AKT2 inhibited inhibitor of κB phosphorylation. In brief, miR­625­5p may protect LPS­induced HBECs by targeting AKT2 and inhibiting the nuclear factor­κB signaling pathway. Therefore, miR­625­5p may function as an inhibitor of asthma airway inflammation in HBECs by targeting AKT2.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Brônquios / MicroRNAs / Proteínas Proto-Oncogênicas c-akt / Inflamação Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Brônquios / MicroRNAs / Proteínas Proto-Oncogênicas c-akt / Inflamação Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article