Gene expression profiling of porcine skeletal muscle satellite cells after poly(I:C) stimulation.
Gene
; 695: 113-121, 2019 May 05.
Article
em En
| MEDLINE
| ID: mdl-30633943
ABSTRACT
Porcine satellite cells (PSCs) play a vital role in the construction, development and self-renewal of skeletal muscle. In this study, PSCs were exposed to poly(IC) stimulation to mimic viral infection during the proliferation and differentiation phases at 0, 12, 24 and 48â¯hours (h) of the stimulation. The untreated and treated PSCs were analyzed by the RNA-Seq technology. There were 88, 119, 104 and 95 genes being differentially expressed in 0â¯h vs 12â¯h treated, 12â¯h vs 24â¯h treated, 0â¯h vs 24â¯h treated and 24â¯h vs 48â¯h untreated comparison libraries, respectively. The GO terms analysis results showed that during the proliferation phase of treated PSCs, the up-regulated genes related to the immune system were highly expressed. In addition, the gene expressions associated with muscle structure development in response to growth factor emerged during the differentiation phase of untreated PSCs. The biological pathways associated with Influenza A, Toll-like receptor and chemokine signaling were revealed in PSCs following poly(IC) stimulation. The differentially expressed genes were confirmed by quantitative real-time PCR. These findings expanded our understanding of gene expressions and signaling pathways about the infiltrated mechanism of the virus into PSCs.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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Músculo Esquelético
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Células Satélites de Músculo Esquelético
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Proliferação de Células
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article