The role of osteomodulin on osteo/odontogenic differentiation in human dental pulp stem cells.
BMC Oral Health
; 19(1): 22, 2019 01 22.
Article
em En
| MEDLINE
| ID: mdl-30670012
ABSTRACT
BACKGROUND:
Extracellular matrix secretion and odontoblastic differentiation in human dental pulp stem cells (hDPSCs) are the cellular bases for reparative dentinogenesis. Osteomodulin (OMD) is a member of the small leucine-rich proteoglycan family distributed in the extracellular matrix but little is known about its role in osteo/odontogenic differentiation. The objective of this study was to investigate the role of OMD during osteo/odontoblastic differentiation of hDPSCs.METHODS:
hDPSCs were selected using immune-magnetic beads and their capability of multi-differentiation was identified. OMD knockdown was achieved using short hairpin RNA (shRNA) lentivirus and was confirmed by western blot. Gene expression was measured by real-time qPCR and osteo/odontoblastic differentiation of hDPSCs was determined by alizarin red S staining.RESULTS:
Compared with uninduced cells, the transcription of OMD was up-regulated by 35-fold at the late stage of osteo/odontogenic differentiation. shRNA-mediated gene silencing of OMD decreased the expression of odontoblastic genes, such as alkaline phosphatase (ALP), dentin matrix acidic phosphoprotein 1 (DMP1) and dentin sialophosphoprotein (DSPP). Besides, knockdown of OMD attenuated the mineralized nodules formation induced by osteo/odontogenic medium.CONCLUSIONS:
These results implied that OMD may play a pivotal role in modulating the osteo/odontoblastic differentiation of hDPSCs.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
Proteínas da Matriz Extracelular
/
Polpa Dentária
/
Odontoblastos
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article