Electrooptical determination of the isoelectric point of globular proteins: Cytochrome c adsorbed on montmorillonite nanoplates.
Colloids Surf B Biointerfaces
; 176: 480-487, 2019 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-30684904
ABSTRACT
The purpose is to study the capability of the electric light scattering to determine the point of zero charge of native protein macromolecules adsorbed on colloid particles at low ionic strength and without using buffers. The chosen protein and particles are cytochrome c (cytC, globular haemoproteid) and montmorillonite (MM, negatively charged plate-like crystal). The pH-dependence of the electric polarizability γ(pH) in the range pH 6-11 shows minimum which coincides with the isoelectric point determined by measuring the electrophoretic mobility µ(pH) of the cytC-MM particles.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Bentonita
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Citocromos c
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Eletricidade
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Nanopartículas
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Óptica e Fotônica
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article