Directed Evolution of a Bacterial Laccase (CueO) for Enzymatic Biofuel Cells.
Angew Chem Int Ed Engl
; 58(14): 4562-4565, 2019 03 26.
Article
em En
| MEDLINE
| ID: mdl-30689276
ABSTRACT
Escherichia coli's copper efflux oxidase (CueO) has rarely been employed in the cathodic compartment of enzymatic biofuel cells (EBFCs) due to its low redox potential (0.36â
V vs. Ag/AgCl, pHâ
5.5) towards O2 reduction. Herein, directed evolution of CueO towards a more positive onset potential was performed in an electrochemical screening system. An improved CueO variant (D439T/L502K) was obtained with a significantly increased onset potential (0.54â
V), comparable to that of high-redox-potential fungal laccases. Upon coupling with an anodic compartment, the EBFC exhibited an open-circuit voltage (Voc ) of 0.56â
V. Directed enzyme evolution by tailoring enzymes to application conditions in EBFCs has been validated and might, in combination with molecular understanding, enable future breakthroughs in EBFC performance.
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Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Fontes de Energia Bioelétrica
/
Proteínas de Escherichia coli
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article