Dietary chitosan-selenium nanoparticle (CTS-SeNP) enhance immunity and disease resistance in zebrafish.
Fish Shellfish Immunol
; 87: 449-459, 2019 Apr.
Article
em En
| MEDLINE
| ID: mdl-30703551
ABSTRACT
Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20⯵g/g but the best outcome was observed at 10⯵g/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10⯵g/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Selênio
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Peixe-Zebra
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Quitosana
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Nanopartículas
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Resistência à Doença
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Doenças dos Peixes
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Imunidade Inata
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article